C.E. Bennett Research pages Back to 'Ecto- and Endo- Parasites'
Services to Industry :
Major Opportunities exist in marketing through liaison.
Film production, script writing and presenting is available to Pharmaceutical Industry and Educational Establishements
See for example collaboration with the EMMY award winning film maker David Barlow.
There is access to free-living stages, parasite eggs, larvae or in-vitro cultured parasites.
Services include development work on parasitological methods including parasite detection and drug resistance testing.
Novel pharmacological screens include image analysis of protozoa, helminths and arthropods. Molecular and Cell Biology are used to advance anti-parasitic vaccines including liver fluke and Proliferative Kidney Disease in Rainbow Trout. With students we traced the source of infection in the river Itchen to bryozoa in culverts.
We have links to The Ministry of Agriculture Fisheries and Foods at the Central Veterinary laboratories Weybrige and the Fish Diseases Lab at Weymouth (Dr. Steve Feist).
Drug Screening Methods and Services
Businesses and those involved in research who are interested in our services of compound screening methodologies please contact us at the below e-mail address for further details. We offer a range of helminth screening methods. Rates can be negiotiated
Worm Breeder's Gazette 15(3): 13 (June 1, 1998)
N.B. These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal
communication and should be cited as such only with the consent of the author.
Clive Bennett E-mail: firstname.lastname@example.org
Video analysis of drug-modulated behaviour in adult
Andrew E Williams, David J Brownlee, Clive E Bennett
Division of Cell Sciences, School of Biological Sciences,
University of Southampton, Southampton, SO16 7PX,
The increase of drug resistance in nematodes has generated an interest in developing novel compounds to control both animal and
plant parasitic species. Caenorhabditis elegans has many advantages enabling its use as a pharmacological screen. It is easily
maintained within the laboratory, has a short life cycle, and can be used as a model for animal and plant parasitic nematodes in
addition to nematode locomotory behaviour. This has lead us to investigate the potential of video analysis to determine the specific
effects that such compounds may have on nematode locomotory behaviour by using C. elegans . Previous studies on parasitic
nematodes have concentrated upon standard in vitro assays (LD50s: concentration which produce 50% mortality) and not
pharmacological based behavioural studies per se.
Adult C. elegans were incubated in a range of common anthelmintic compounds (e.g. levamisole, piperazine) and
neurotransmitters ("classical" and FaRPs), and the resulting behavioural changes recorded onto video-tape. Resultant data analysis,
was performed by an image-analysis software package (statistical analysis (mean +/- s.e.mean) by Graphpad Prism) which was
used to resolve the behavioural attributes of C. elegans locomotory behaviour (distance moved (micro-m), time moving/still (secs),
total speed (micro-m/sec), and degrees/second). Concentration dependent effects were observed with piperazine and other drugs.
Piperazine caused a statistically significant reduction in total distance moved, total speed, and degrees/sec. This study has shown
that this method of analysis is an effective broad-based in vitro pharmacological screen for compounds with a neuromuscular
mode of action.
© Dr. Clive Bennett and Dr. D.J. A. Brownlee 1998